FGFR expression levels of the MRT cell lines A204, G401 and G402.

(A) Scatter plot showing expression and copy number levels for FGFR1 (left panel) and FGFR2 (right panel) within the CCLE. MRT lines A204, G401 and G402 are indicated in red. (B) Quantitative RT-PCR (qRT-PCR) analysis of FGFR1 and FGFR2 mRNA expression in MRT cell lines and soft tissue cancer lines SKLMS1 and SKUT1. Expression values are given as average with standard errors of the mean (SEM) (n≥3) with respect to GAPDH mRNA levels (arbitrarily set as 100). (C) qRT-PCR and immunoblot analysis of SNF5-deficiency in MRT lines. SKLMS1 and SKUT1 cells were used as positive controls for SNF5 expression. SNF5 mRNA expression is given as average with SEM (n≥3) with respect to GAPDH mRNA levels (arbitrarily set as 100). β-Tubulin expression was used to monitor equal loading.(A) 散点图展示了癌症细胞系百科（Cancer Cell Line Encyclopedia, CCLE）中成纤维细胞生长因子受体1（Fibroblast Growth Factor Receptor 1, FGFR1，左侧面板）与成纤维细胞生长因子受体2（Fibroblast Growth Factor Receptor 2, FGFR2，右侧面板）的表达水平及拷贝数水平。恶性横纹肌样瘤（Malignant Rhabdoid Tumor, MRT）细胞系A204、G401与G402以红色标注。 (B) 针对MRT细胞系与软组织癌细胞系SKLMS1、SKUT1，开展了FGFR1与FGFR2的mRNA表达水平定量逆转录聚合酶链反应（quantitative Reverse Transcription Polymerase Chain Reaction, qRT-PCR）分析。表达值以相对于甘油醛-3-磷酸脱氢酶（Glyceraldehyde-3-Phosphate Dehydrogenase, GAPDH）mRNA水平的平均值±标准误（Standard Error of the Mean, SEM）表示，任意设定GAPDH的表达水平为100，样本量n≥3。 (C) 针对MRT细胞系开展了SNF5（SWI/SNF相关基质相关肌动蛋白调控染色质因子5，SWI/SNF-related Matrix-associated Actin-dependent Regulator of Chromatin 5）缺失情况的qRT-PCR与免疫印迹（immunoblot）分析。以SKLMS1与SKUT1细胞作为SNF5表达的阳性对照。SNF5的mRNA表达值以相对于GAPDH mRNA水平的平均值±标准误表示，任意设定GAPDH的表达水平为100，样本量n≥3。以β微管蛋白（β-Tubulin）的表达作为上样量均一性的检测指标。