Translation efficiency in the mouse liver upon 24h fasting

To investigate the translatome in the fasted condition and the role of phosphoralated eIF4E in it We performed polysome sequencing on fed and fasted wild type mice and fasted eIF4E S209A/S209A mice Overall design: We seperated highly translated mRNA and low translated mRNA using sucrose gradient and perform comparative gene expression profiling analysis of RNA-seq data for those high tanslated and low translated mRNA from fed and fasted WT livers, and fasted eIF4E S209A/S209A livers