Supplementary Material for: Single Nucleotide Polymorphisms in Adiponectin Gene Are Not Directly Associated with Increased Risk of Obstructive Sleep Apnea Syndrome in a Chinese Han Population

<i>Purpose:</i> This study aims to test the possible correlation between single nucleotide polymorphisms (SNPs) in the adiponectin gene and increased risk of obstructive sleep apnea syndrome (OSAS) in a Chinese Han population. <i>Materials and Methods:</i> A total of 266 subjects were enrolled into the study to detect 9 SNPs in the adiponectin gene. Multivariate unconditional logistic regression analysis, adjusted for gender and age, was used to estimate the associations of these SNPs with OSAS risk. <i>Results:</i> No evidence of a direct association was observed between these SNPs and the risk of OSAS in the Chinese Han population. However, the stratified analysis also revealed a remarkable genotype difference for SNP rs6773957 between cases and controls in the overweight subgroup (<i>p </i>&lt; 0.05). In addition, the allele or genotype distributions of rs12495941, rs182052, and rs16861205 had significant differences with regard to the severity of OSAS (<i>p </i>&lt; 0.05). No differences were identified in the other subgroups. <i>Conclusion:</i> The current research demonstrated that the SNPs in the adiponectin gene did not represent susceptibility loci for OSAS in Chinese Han individuals overall. However, variants of rs6773957 have an association with OSAS in overweight individuals. In addition, polymorphisms of rs12495941, rs182052, and rs16861205 are associated with the severity of OSAS.

研究目的：本研究旨在在中国汉族人群中，探究脂联素基因（adiponectin gene）的单核苷酸多态性（single nucleotide polymorphisms, SNPs）与阻塞性睡眠呼吸暂停综合征（obstructive sleep apnea syndrome, OSAS）发病风险升高之间的潜在相关性。
材料与方法：本研究共纳入266名受试者，对脂联素基因上的9个单核苷酸多态性位点进行检测。采用校正了性别与年龄的多因素非条件logistic回归分析，评估上述单核苷酸多态性与阻塞性睡眠呼吸暂停综合征发病风险的关联。
结果：在中国汉族人群中，未观察到上述单核苷酸多态性与阻塞性睡眠呼吸暂停综合征发病风险存在直接关联的证据。然而，分层分析显示，在超重亚组中，SNP rs6773957的基因型在病例组与对照组间存在显著差异（p < 0.05）。此外，rs12495941、rs182052及rs16861205的等位基因或基因型分布与阻塞性睡眠呼吸暂停综合征的严重程度存在显著差异（p < 0.05）。其余亚组未发现相关差异。
结论：本研究表明，总体而言，脂联素基因的单核苷酸多态性并非中国汉族人群阻塞性睡眠呼吸暂停综合征的易感位点。但rs6773957的变异与超重人群的阻塞性睡眠呼吸暂停综合征存在关联。此外，rs12495941、rs182052及rs16861205的多态性与阻塞性睡眠呼吸暂停综合征的严重程度相关。