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Additional file 3 of Characterization of the plant homeodomain (PHD) reader family for their histone tail interactions

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Additional file 3: Table S2. Histone Peptide Microarray Results. Excel Tab Peptide List is a compiled list of the entire 293 histone peptide library with amino acid sequence ranges. Excel Tab Peptide Array Grid Map is a table representing the pattern in which peptides have been printed for the peptide microarray. Each number corresponds to the peptide number designated in Peptide List. Excel Tab Average Signals is a compiled set of binding data: chromatin reader at 0.5 μM vs. entire 293 peptide library. Data is presented as the average and standard deviation of 4 replicates. The average signals are colored to indicate signal intensity with green being strong and white being weak/low. Excel Tab Array Heatmap contains a condensed form of Average Signals data displaying an average of 4 replicates for each chromatin reader tested and individually formatted to demonstrate binding specificity. Key: red= stronger relative binding; blue = weaker relative binding. Excel Tab Array info summary is a table summarizing the domains, sequence coverage, array signal range, and noise for each of the GST-tagged readers assayed on the peptide microarrays.

附加文件3:表S2。组蛋白肽微阵列(Histone Peptide Microarray)实验结果。 Excel工作表"肽段列表"收录了完整的293个组蛋白肽段文库的氨基酸序列范围信息。 Excel工作表"肽微阵列网格图谱"用于展示肽微阵列的肽段点样布局,其中每个数字均对应"肽段列表"中指定的肽段编号。 Excel工作表"平均信号"为整合后的结合实验数据:浓度为0.5微摩尔的染色质阅读器(chromatin reader)与完整的293个肽段文库的结合数据。数据以4次重复实验的平均值与标准差呈现,平均信号值通过颜色标注信号强度:绿色代表强信号,白色代表弱/低信号。 Excel工作表"阵列热图"为"平均信号"数据的精简版本,展示了每个被测染色质阅读器的4次重复实验平均值,并经过专属格式化以直观呈现结合特异性。图例说明:红色代表相对结合强度更高,蓝色代表相对结合强度更低。 Excel工作表"阵列信息汇总"汇总了在肽微阵列上完成检测的每个带谷胱甘肽S-转移酶标签(GST-tag)的阅读器的结构域、序列覆盖度、阵列信号范围及噪音水平。

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2020-01-25
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