Allogeneic mesenchymal stem cells and xenogenic platelet rich plasma, associated or not, in the repair of bone failures in rabbits with secondary osteoporosis

Abstract Purpose: To assess the efficacy of allogeneic mesenchymal stem cells and xenogenic platelet rich plasma in the treatment of bone failure of osteoporotic rabbits secondary to estrogenic deprivation and iatrogenic hypercortisolism. Methods: Eight female rabbits underwent ovarian resection and corticoid therapy to induce clinical status of osteoporosis. Four failures were produced in the tibiae, with each failure being treated with hemostatic sponge, allogenic mesenchymal stem cells, xenogenic platelet-rich plasma and the association between both. The animals were divided into two groups, evaluated radiographically and histopathologically at 30 and 60 days post treatment. Results: A radiographically confirmed consolidation of bone failures treated with allogeneic mesenchymal stem cells, associated with the histopathological image of mature and immature bone tissue, without evidence of osteopenia, was compared with the other groups, in which radiolucent failures with osteopenia and fibrosis were still present, denoting the satisfactory effect of the first treatment in detriment to the others. Conclusion: The treatment of bone failures of rabbits with secondary osteoporosis with allogeneic mesenchymal stem cells induced greater bone consolidation with mature and immature bone tissue production (p<0.01), when compared to the other treatments.

摘要
目的：评估同种异体间充质干细胞（allogeneic mesenchymal stem cells）与异种富血小板血浆（xenogenic platelet-rich plasma）治疗继发于雌激素剥夺及医源性皮质醇增多症的骨质疏松家兔骨缺损的疗效。
方法：选取8只雌性家兔，通过卵巢切除术联合糖皮质激素治疗构建继发性骨质疏松模型；于其胫骨处制备4处骨缺损，分别以止血海绵、同种异体间充质干细胞、异种富血小板血浆以及二者联合方案进行治疗。所有实验动物均于治疗后30天、60天两个时间点，开展影像学及组织病理学评估。
结果：影像学检查证实，接受同种异体间充质干细胞治疗的骨缺损均实现骨愈合，组织病理学可见成熟与未成熟骨组织形成，无骨质减少征象；而其余各组仍存在透光性骨缺损，伴骨质减少与纤维化，提示该治疗方案的疗效优于其余组别。
结论：与其余治疗方案相比，同种异体间充质干细胞治疗继发性骨质疏松家兔骨缺损可促进更充分的骨愈合，生成成熟与未成熟骨组织，差异具有统计学意义（p<0.01）。