The changed piRNA profiles of GC-1 cells exposure to 500 nM MC-LR.. Mus musculus

We treated GC-1 cells with 500 nM or 0 nM of MC-LR for 24 h.Next, total RNA from GC-1 cells of these two groups were extracted using TRIzol Reagent. The two small RNA libraries were prepared using the Illumina small RNA preparation kit and were deep-sequenced. After adapter sequences were trimmed, 10,824,057, and 11,566,902 small RNA reads that were smaller than 45 nucleotides were obtained from control group and MC-LR group, respectively. The sequences that correspond to known piRNAs were determined by perfect sequence matching to the NCBI database (http://www.ncbi.nlm.nih.gov). We identified 244066 reads of piRNAs and 220 unique piRNA sequences in control group and 359871 reads of piRNAs and 230 unique piRNA sequences in MC-LR group Overall design: The piRNA profiles of GC-1 cells exposure to 500 nM or 0 nM MC-LR were generated by Illumina high-throughput sequencing.