Next Generation Sequencing Analysis of Prenatal Chlorpyrifos Exposure embryonic kidneys

Purpose: In order to study signaling pathway changes of prenatal chlorpyrifos exposure embryonic kidney development Methods: By using RNA-seq, we studied the transcriptome of E12.5, E14.5, E16.5 and E18.5 prenatal chlorpyrifos exposure embryonic kidneys and DMSO exposure (control) embryonic kidneys Results: We show that Notch signaling pathway and aquaporin family had high expression in chlorpyrifos exposure E18.5 kidney. The nephron progenitor markers had low expression in chlorpyrifos exposure embryonic kidneys Overall design: ICR mice aged 8 weeks were bred using timed matings, the pregnant mice were randomly divided into two groups. Chlorpyrifos (96% purity) was dissolved in dimethyl sulfoxide (DMSO), and CPF 5mg/kg was injected subcutaneously daily in CPF group during gestation day 7.5-11.5. The controls were injected with isovolumetric DMSO at the same time. We collected E12.5, E14.5, E16.5 and E18.5 mouse embryonic kidneys for RNA-seq of the two groups respectively.