Flow cytometric analysis of cell surface expression of STRO-1, CD105, CD166 and CD90.

One million PDL cells were analyzed with antibodies against the human antigens STRO-1, CD105, CD166 and CD90 by flow cytometry. Positive expression was defined as the level of fluorescence greater than 99% of the corresponding isotype-matched control antibodies. Data represent (%) mean ± standard deviation (SD) (n = 3).采用流式细胞术（flow cytometry）对100万个牙周膜细胞（Periodontal Ligament cells，PDL）进行检测，所用抗体靶向人源抗原STRO-1、CD105、CD166与CD90。阳性表达的判定标准为荧光强度高于对应同型匹配对照抗体的99%水平。数据以百分比形式呈现为平均值±标准差（SD），实验重复3次（n=3）。