MiRNA expression-based, individualized outcome prediction for surgically paired solitary and multiple leiomyoma with its corresponding myometrium

MiRNA microarray analysis was performed on 12 tissues from surgical leiomyoma patients, including three pairs of solitary leiomyoma and its corresponding myometrium and three pairs of multiple leiomyomas and corresponding myometrium. MiRNA microarray analysis was performed on 12 tissues from surgical leiomyoma patients. Tissues of uterine leiomyomas and corresponding normal myometrium were collected from 6 women with symptomatic leiomyomas, who underwent operations at West China Second University Hospital (Chengdu, P.R. China), and were stored in liquid nitrogen immediately after surgeries. Among 6 patients, 3 were diagnosed with solitary leiomyoma and 3 were multiple according to surgical findings.Written informed consents were obtained from all patients, and the Institutional Review Board of West China Second University Hospital approved the study protocol.During tissue collection, eligible subjects and tissue specimens were defined as: 1) no GnRH-a or oral contraception used before surgery; 2) pathological diagnosis of leiomyoma; 3) no accompanying adenomyosis or endometriosis; 4) minimum number of leiomyomas involved in multiple leiomyomas was 5; and 5) leiomyomas intraoperatively revealed as multiple leiomyomas fused as one were excluded from solitary leiomyoma group. 6) SL was defined as one leiomyomas identified by both ultrasound and surgery findings. And the diameter of SL should be larger than 8cm.