Fig2E_NB_luciferase_R3_LG379.tif

HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with a luciferase reporter with or without a 99bp sequence from the STOML2 gene inserted. RNA was extracted and examined by northern blotting. Ethidium bromide-stained gel images are also included to show rRNA.

将HEK293T ishXrn1细胞用多西环素（doxycycline）处理3~4天以诱导Xrn1基因敲低，随后转染携带或不携带STOML2基因来源的99bp插入序列的荧光素酶报告基因载体。提取RNA并通过Northern印迹（Northern blotting）进行检测，同时附上溴化乙锭染色的凝胶图像以展示核糖体RNA（rRNA）。