Single-cell RNA-seq profiles of lung adenocarcinoma patients and tumor-bearing mice

single-cell RNA sequencing (scRNA-seq) profiles from eight patients with lung adenocarcinoma (LUAD) and four samples of tumor tissues from tumor bearing mice were performed. By integrating other scRNA-seq data and clinical information, we identified activated adaptive immune responses in older patients, reflected by enriched dysfunctional T cell signature scores and immune checkpoint molecules. Our study shows increased efficacy of immune checkpoint blockade therapy in older patients, addressing the prominent role of age when considering immunotherapy.本研究对8例肺腺癌（lung adenocarcinoma，LUAD）患者及4份荷瘤小鼠的肿瘤组织样本开展了单细胞RNA测序（single-cell RNA sequencing，scRNA-seq），获得了对应的表达谱数据。通过整合其他单细胞RNA测序数据与临床信息，本研究鉴定出老年患者体内存在激活的适应性免疫应答，该应答以功能失调性T细胞特征评分富集以及免疫检查点分子高表达为典型特征。本研究证实老年患者的免疫检查点阻断治疗疗效更优，明确了年龄在免疫治疗决策中的核心地位。