Datapoints of Fig 2.

Medaka fish (Oryzias latipes) is a powerful model to study genetics underlying the developmental and functional traits of the vertebrate visual system. We established a simple and high-throughput optomotor response (OMR) assay utilizing medaka larvae to study visual functions including visual acuity and contrast sensitivity. Our assay presents multiple adjustable stripes in motion to individual fish in a linear arena. For that the OMR assay employs a tablet display and the Fish Stripes software to adjust speed, width, color, and contrast of the stripes. Our results demonstrated that optomotor responses were robustly induced by black and white stripes presented from below in the linear-pool-arena. We detected robust strain specific differences in the OMR when comparing long established medaka inbred strains. We observed an interesting training effect upon the initial exposure of larvae to thick stripes, which allowed them to better respond to narrower stripes. The OMR setup and protocol presented here provide an efficient tool for quantitative phenotype mapping, addressing visual acuity, trainability of cortical neurons, color sensitivity, locomotor response, retinal regeneration and others. Our open-source setup presented here provides a crucial prerequisite for ultimately addressing the genetic basis of those processes.青鳉（Oryzias latipes）是研究脊椎动物视觉系统发育与功能性状遗传基础的经典实验模型。我们建立了一套简便高通量的视动反应（optomotor response, OMR）检测方法，利用青鳉幼体开展视觉功能研究，涵盖视敏度与对比敏感度等核心指标。该检测系统可在线性槽式装置内为单条幼鱼呈现多组可调节的运动条纹，通过平板显示器与Fish Stripes软件，可精准调控条纹的运动速度、宽度、颜色与对比度。实验结果表明，在该装置中由下方投射的黑白条纹可稳定诱发出视动反应。通过比对多个长期传代的青鳉近交品系，我们发现不同品系的OMR表现存在显著的特异性差异。此外，我们还观察到一项有趣的训练效应：幼鱼初次接触宽条纹后，对窄条纹的响应能力会得到显著提升。本研究提供的OMR装置与实验方案，可作为高效工具用于定量表型定位，覆盖视敏度、皮层神经元可训练性、颜色敏感度、运动响应、视网膜再生等多个研究方向。本研究公开的开源装置，为最终解析上述过程的遗传基础提供了关键前提条件。