Summary data for 22 nonsynonymous SNPs and one putative nonsense SNP predicted to impact protein function.

aSNPs with “rs” numbers were previously described [23]-[25], [30], [59] and validated in this study.
bAmino acid (AA) substitutions predicted from corresponding SNPs, GenBank Proteins, and previous studies [23]-[25], [30], [60].
cProtein domain locations predicted by SMART (http://smart.embl-heidelberg.de/). Only confidently predicted domains are depicted (NCP = no confident prediction; LRRs are named in order of prediction).
dResults from PolyPhen and SIFT [38]-[39]. Results other than “Benign (B)” or “Tolerated (T)” are predicted to be Possibly Damaging (PsD), Probably Damaging (PrD), or Affect Protein Function (AF). SIFT could not be used (ND) to model the TLR5 putative nonsense SNP.
eObserved frequency of nonsynonymous SNP allele across all 31 cattle breeds.
fThe frequency of this SNP in U.S. dairy cattle (n = 405, 3 Herds) was 0.068.