CIL:45836, Homo sapiens. In Cell Image Library

Plate 25403 - Phenotypic profiling attempts to summarize multiparametric, feature-based analysis of cellular phenotypes of each sample so that similarities between profiles reflect similarities between samples. This image set provides a basis for testing image-based profiling methods wrt. to their ability to distinguish the effects of small molecules.

Images
The images are of U2OS cells treated with each of 1600 known bioactive compounds and labeled with six labels that characterize seven organelles (the .cell-painting. assay).

This pilot experiment consists of 20 plates. Each plate has 384 wells and each well has 9 fields of view for a total of 69,120 fields of view. Each field was imaged in five channels (detection wavelengths), and each channel is stored as a separate, grayscale image file, so there are 345,600 image files in 16-bit TIFF format.

编号25403的检测板：表型谱分析（Phenotypic profiling）旨在通过多参数、基于特征的分析手段，对每个样本的细胞表型进行归纳总结，使得谱图间的相似性能够反映样本间的相似性。本图像集为基于图像的谱分析方法提供了测试基准，用于评估其区分小分子化合物作用效果的能力。
图像
本次成像的样本为经1600种已知生物活性化合物分别处理的U2OS细胞，通过六种标记物对七种细胞器进行染色标记（即细胞绘画检测法（cell-painting assay））。
本次预实验共包含20块检测板。每块检测板设有384个培养孔，每个培养孔包含9个视场，总计69120个视场。每个视场通过五个通道（检测波长）完成成像，每个通道均以独立的灰度图像文件存储，因此本数据集共包含345600个16位TIFF格式的图像文件。