Genomewide measurements of neural-specific mRNA decay in Drosophila embryos. Drosophila melanogaster

Neural-specific mRNA decay measurements by TU-Decay technique in control and Pumilio knockdown embryos Overall design: These TU-Decay microarrays analyze mRNA levels at three timepoints: a one hour pulse, one hour chase, and three hour chase. Neural-specific RNA purification was achieved using prospero-GAL4 driving UAS-T.g.UPRT. Pumilio knockdown in the nervous system was acheived using UAS-Pum(RNAi) driven by prospero-Gal4.