Binding site size limit of the 2:1 pyrrole-imidazole polyamide-DNA motif.
收藏PubMed Central1996-07-09 更新2026-05-02 收录
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https://pmc.ncbi.nlm.nih.gov/articles/PMC38920/
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资源简介：
Polyamides containing N-methylimidazole (Im) and N-methylpyrrole (Py) amino acids can be combined in antiparallel side-by-side dimeric complexes for sequence-specific recognition in the minor groove of DNA. Six polyamides containing three to eight rings bind DNA sites 5-10 bp in length, respectively. Quantitative DNase I footprint titration experiments demonstrate that affinity maximizes and is similar at ring sizes of five, six, and seven. Sequence specificity decreases as the length of the polyamides increases beyond five rings. These results provide useful guidelines for the design of new polyamides that bind longer DNA sites with enhanced affinity and specificity. IMAGES:
提供机构：
National Academy of Sciences
创建时间：
1996-07-09
