CIL:38652

This image combines total internal reflection microscopy (TIRF) of mCerulean-actin (blue) with photoactivation localization microscopy (PALM) image of tdEos-vinculin (red) and Dronpa-paxillin (green). Vinculin and paxillin are organized into focal adhesions at the ends of actin bundles. A higher magnification of several adhesion complexes (CIL 38651) clearly shows that vinculin and paxillin are segregated into interlocking microdomains within focal adhesions. Bar is 2 microns. Image made available by Catherine and James Galbraith and corresponds to Figure 5 in PNAS U S A. 2007 Dec 18;104(51):20308-13.

本图像将mCerulean标记的肌动蛋白（mCerulean-actin，蓝色）的全内反射显微镜（total internal reflection microscopy, TIRF）成像结果，与tdEos标记的纽蛋白（tdEos-vinculin，红色）和Dronpa标记的桩蛋白（Dronpa-paxillin，绿色）的光激活定位显微镜（photoactivation localization microscopy, PALM）成像结果进行了融合。纽蛋白与桩蛋白在肌动蛋白束末端的黏着斑（focal adhesions）中形成有序排布。对数个黏着复合体（CIL 38651）进行高倍放大后可清晰观察到，纽蛋白与桩蛋白在黏着斑内部被分隔为相互嵌合的微结构域。图像标尺长度为2微米。本图像由Catherine与James Galbraith提供，对应《美国国家科学院院刊（Proceedings of the National Academy of Sciences USA, PNAS USA）》2007年12月18日刊发的第104卷第51期第20308-20313页的图5。