Bisulfite Genome for hg19 with phage lambda genome

The data provided here is an indexed version of the human genome hg19. The hg19 fasta file was originally downloaded from UCSC (http://hgdownload.soe.ucsc.edu/goldenPath/hg19/bigZips/hg19.fa.gz) and combined with a target portion of the phage lambda genome from 4500 to 6500 base pairs, originally downloaded from NCBI (https://www.ncbi.nlm.nih.gov/nuccore/NC_001416.1.fa.gz)The Bismark program to map bisulfite treated sequencing reads was used to generate the index using the bismark_genome_preparation command. The indexed files then compressed using tar and then pigz to make a small and. Ordered set of the Bisulfite Genome files.References:<br> Felix Krueger, Simon R. Andrews, Bismark: a flexible aligner and methylation caller for Bisulfite-Seq applications, <em>Bioinformatics</em>, Volume 27, Issue 11, 1 June 2011, Pages 1571–1572, https://doi.org/10.1093/bioinformatics/btr167本数据集为人类参考基因组hg19的索引版本。原始hg19 FASTA格式文件下载自UCSC（http://hgdownload.soe.ucsc.edu/goldenPath/hg19/bigZips/hg19.fa.gz），并并入了噬菌体λ基因组4500至6500碱基对区间的目标序列片段，该噬菌体序列原始下载自NCBI（https://www.ncbi.nlm.nih.gov/nuccore/NC_001416.1.fa.gz）。 本数据集使用用于比对亚硫酸氢盐处理测序读段的Bismark工具，通过bismark_genome_preparation命令生成基因组索引；随后将索引文件通过tar与pigz工具压缩，得到体积小巧且排列有序的亚硫酸氢盐基因组文件集。 参考文献：<br> Felix Krueger、Simon R. Andrews，《Bismark：面向亚硫酸氢盐测序（Bisulfite-Seq）应用的灵活比对器与甲基化调用工具》，*Bioinformatics*，第27卷第11期，2011年6月1日，第1571-1572页，https://doi.org/10.1093/bioinformatics/btr167