Frequency of EBV latent antigen-specific antibody responses in HC, MS and POST-IM donors.

Donor plasma was probed for antibody reactivity to lysates from C2 cells infected with type 1 (T1) or type 2 (T2) EBV strains by Western blot. Reactivity to EBV latent antigens was determined by bands of the corresponding size as described in the methods section. For analysis of antibody responses to EBNA3 antigens, cell lysates from BJAB cells were infected separately by MVA viruses expressing individual EBNA3A, B and C proteins and antibody reactivity determined by bands corresponding to the correct size.