Data from: Topological, chemical, and electro-optical characteristics of riboflavin-doped artificial and natural DNA thin films

DNA is considered as a useful building bio-material, and it serves as an efficient template to align functionalized nanomaterials. Riboflavin (RF)-doped synthetic double-crossover DNA (DX-DNA) lattices and natural salmon DNA (SDNA) thin films are constructed using substrate assisted growth and drop-casting methods, respectively, and their topological, chemical, and electro-optical characteristics were evaluated. The critical doping concentration of RF ([RF]C, ~5 mM) at given concentrations of DX-DNA and SDNA were obtained by observing the phase transition (from crystalline to amorphous structures) of DX-DNA and precipitation of SDNA in solution above [RF]C. [RF]C are verified by analyzing the atomic force microscopy images for DX-DNA and current, absorbance, and photoluminescence for SDNA. We study the physical characteristics of RF-embedded SDNA thin films, which are the Fourier transform infrared (FTIR) spectrum to understand the interaction between the RF and DNA molecules, current to evaluate the conductance, absorption to understand the RF binding to the DNA, and photoluminescence (PL) to analyze the energy transfer between the RF and DNA. The current and UV absorbance band of SDNA thin films decrease up to [RF]C followed by an increase above [RF]C. In contrast, the PL intensity illustrates the reverse trend, as compared to the current and UV absorbance behavior as a function of the varying [RF]. Due to the intense PL characteristic of RF, the DNA lattices and thin films with RF might offer immense potential to develop efficient bio-sensors and useful bio-photonic devices.

DNA被视为一种极具应用价值的功能性生物构建材料，可作为高效模板用于排列功能化纳米材料。本研究分别通过衬底辅助生长法与滴涂法制备了掺核黄素（Riboflavin, RF）的合成双交叉DNA（DX-DNA）晶格与天然鲑鱼DNA（SDNA）薄膜，并对其拓扑结构、化学特性与电光性能进行了表征评估。在固定DX-DNA与SDNA浓度的条件下，通过观察DX-DNA的相转变（从晶态转变为非晶态）以及溶液中SDNA在临界掺杂浓度[RF]C（约5 mM）以上时的沉淀现象，确定了该临界掺杂浓度。针对DX-DNA，通过原子力显微镜（AFM）图像分析验证其[RF]C阈值；针对SDNA，则通过电流、吸光度与光致发光（PL）表征完成验证。本研究还探究了嵌入RF的SDNA薄膜的物理特性：利用傅里叶变换红外（FTIR）光谱分析RF与DNA分子间的相互作用，通过电流评估其导电性能，借助吸光度表征RF与DNA的结合情况，并利用光致发光（PL）分析RF与DNA之间的能量传递过程。SDNA薄膜的电流与紫外吸光度随RF浓度变化呈现先下降至[RF]C、随后在[RF]C以上逐渐升高的趋势；与之相反，PL强度则呈现出与电流、紫外吸光度完全相反的变化规律。鉴于RF具有优异的光致发光特性，掺RF的DNA晶格与薄膜在开发高效生物传感器与实用生物光子器件方面具有巨大应用潜力。