Zoonotic infection of Brazilian primate workers with New World simian foamy virus

Simian foamy viruses (SFVs) are retroviruses present in nearly all nonhuman primates (NHPs), including Old World primates (OWP) and New World primates (NWP). While all confirmed human infections with SFV are from zoonotic transmissions originating from OWP, little is known about the zoonotic transmission potential of NWP SFV. We conducted a longitudinal, prospective study of 56 workers occupationally exposed to NWP in Brazil. Plasma from these workers was tested using Western blot (WB) assays containing NWP SFV antigens. Genomic DNA from blood and buccal swabs was analyzed for the presence of proviral SFV sequences by three nested PCR tests and a new quantitative PCR assay. Exposure histories were obtained and analyzed for associations with possible SFV infection. Ten persons (18%) tested seropositive and two persons were seroindeterminate (3.6%) for NWP SFV. Six persons had seroreactivity over 2-3 years suggestive of persistent infection. All SFV NWP WB-positive workers reported at lea...

猿泡沫病毒（Simian Foamy Viruses, SFV）是一类几乎可感染所有非人灵长类动物（Nonhuman Primates, NHPs）的逆转录病毒，涵盖旧世界猴类（Old World Primates, OWP）与新世界猴类（New World Primates, NWP）。目前所有已确认的人类SFV感染均源自旧世界猴类的人畜共患传播，但学界对新世界猴类SFV的跨物种传播潜力仍知之甚少。本研究针对巴西境内56名职业性暴露于新世界猴类的工作人员开展了一项纵向前瞻性研究：采用搭载新世界猴类SFV抗原的蛋白质印迹（Western Blot, WB）检测方法，对受试者的血浆样本进行检测；同时通过三项巢式PCR实验与新型定量PCR检测方法，分析血液与颊拭子样本中的基因组DNA是否携带前病毒SFV序列。此外，研究人员收集并分析受试者的暴露史，以探究其与SFV感染的潜在关联。研究结果显示，10名受试者（占比18%）的新世界猴类SFV血清学检测呈阳性，另有2名受试者（占比3.6%）血清学结果不确定；其中6名受试者的血清反应性持续2至3年，提示存在持续性感染。所有新世界猴类SFV蛋白质印迹检测阳性的工作人员均报告至少……