Additional file 2: of Systems-level organization of yeast methylotrophic lifestyle

Comparison of gene regulation in P. pastoris cultivated with methanol/glycerol or glucose as carbon source in chemostat to transcriptomics data obtained in fed batch cultivation with methanol glycerol or glucose as carbon source. Average fold changes and P values of regulation patterns for methanol vs glycerol and methanol vs glucose 1 h our after starting the methanol feed are shown. Average values derive from three biological replicates per condition. Containing the following four sheets: Description: Experimental setup of the fed batch cultivations, description, and discussion of observed similarities and differences in gene regulations. Upregulated_methanol: Comparison of all genes upregulated in P. pastoris cultivated with methanol/glycerol or glucose as carbon source in chemostat to transcriptomics data obtained by analyzing methanol vs glycerol and methanol vs glucose fed batches. Downregulated_methanol: Comparison of all genes downregulated in P. pastoris cultivated with methanol/glycerol or glucose as carbon source in chemostat to transcriptomics data obtained by analyzing methanol vs glycerol and methanol vs glucose fed batches. All_data: Transcriptomics data of Additional file 1 (P. pastoris cultivated with methanol/glycerol or glucose as carbon source in chemostat) compared to transcriptomics data obtained by analyzing methanol vs glycerol and methanol vs glucose fed batches. (XLSX 1178 kb)本数据集针对以甲醇/甘油或葡萄糖为碳源恒化培养（chemostat）的巴斯德毕赤酵母（P. pastoris）的基因调控水平，与以甲醇、甘油或葡萄糖为碳源开展补料分批培养（fed batch cultivation）所获取的转录组学数据进行对比分析。本数据集展示了甲醇补料启动1小时后，甲醇与甘油、甲醇与葡萄糖两组基因调控模式的平均倍数变化与P值，所有统计值均来自各实验条件下的三次生物学重复。本数据集包含以下四个工作表： 1. Description：包含补料分批培养的实验设置说明，以及观测到的基因调控模式间相似性与差异性的描述与讨论。 2. Upregulated_methanol：收录以甲醇/甘油或葡萄糖为碳源恒化培养的巴斯德毕赤酵母中全部上调表达基因，并将该数据与甲醇vs甘油、甲醇vs葡萄糖补料分批培养的转录组分析结果进行对比。 3. Downregulated_methanol：收录以甲醇/甘油或葡萄糖为碳源恒化培养的巴斯德毕赤酵母中全部下调表达基因，并将该数据与甲醇vs甘油、甲醇vs葡萄糖补料分批培养的转录组分析结果进行对比。 4. All_data：将附加文件1（以甲醇/甘油或葡萄糖为碳源恒化培养的巴斯德毕赤酵母转录组学数据），与甲醇vs甘油、甲醇vs葡萄糖补料分批培养的转录组分析结果进行对比的完整数据集。本数据集为XLSX格式，文件大小1178 KB。