IPSC-derived brain cells differentiation and characterization.

(A) Protocols for differentiation of IPSC-derived NPC into different brain cell types (neurons, Ast and OLs). (B) Morphology of different brain cell types as phase contrast images at the end of differentiation protocol, (scale bars, 100 μm). (C) Representative positive (as differentiation efficiency markers) and negative (as cell culture purity markers) immunostaining for respective markers for Neurons (NeuN and β-Tubulin as positive staining), OLs (MBP as positive staining) and Ast (GFAP as positive staining) from control cells. (D-E) Representative positive immunostaining for AMN and cALD cells for neuron markers (β-Tubulin), for Ast (GFAP) and for OLs markers (MBP).(A) 诱导多能干细胞（Induced Pluripotent Stem Cell, IPSC）衍生的神经前体细胞（Neural Progenitor Cell, NPC）向神经元、星形胶质细胞（Astrocyte, Ast）及少突胶质细胞（Oligodendrocyte, OLs）等不同脑细胞类型的分化实验方案。(B) 分化流程结束后，各类脑细胞的相差显微镜成像结果（比例尺：100 μm）。(C) 对照细胞中，针对神经元（阳性免疫染色标志物为NeuN与β-Tubulin）、少突胶质细胞（阳性免疫染色标志物为MBP）及星形胶质细胞（阳性免疫染色标志物为GFAP）的代表性阳性免疫染色（作为分化效率标志物）与阴性免疫染色（作为细胞培养纯度标志物）结果。(D)-(E) 肾上腺脊髓神经病（Adrenomyeloneuropathy, AMN）与儿童型脑型肾上腺脑白质营养不良（childhood cerebral adrenoleukodystrophy, cALD）来源细胞中，针对神经元标志物（β-Tubulin）、星形胶质细胞标志物（GFAP）及少突胶质细胞标志物（MBP）的代表性阳性免疫染色结果。