AaCHYMO Forward and Reverse primers used for amplification of the gene of interest without the natural signal leader sequence [12].
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The melting temperature (TM) of each primer was estimated using NetPrimer (Premier Biosoft, Palo Alto, CA). The primers were purchased from ELIM Biopharmaceuticals, Inc. (Hayward, CA). The restriction enzymes for each primer are underlined and in bold.
创建时间：
2025-02-06
