Supplementary Video 1. Feeding a high gain diet to young bulls delays early embryo development.

Cumulus-oocyte complexes were fertilized using semen from bulls fed either a moderate-gain (A) or high-gain (B) diet and cultured individually in a time-lapse incubator. This video shows the in vitro development of bovine embryos from presumptive zygote to hatched blastocyst over ~199 hours post-fertilization. Images were acquired every 5 minutes. The video demonstrates the delayed development and increased cytoplasmic fragmentation of embryos produced from semen of high-gain diet bulls.卵丘-卵母细胞复合体（cumulus-oocyte complexes）采用饲喂中等增重（A组）或高增重（B组）日粮的公牛精液完成受精，并于延时培养箱中单独培养。本视频展示了牛胚胎在受精后约199小时内，从疑似合子阶段发育至孵出囊胚的体外发生过程。图像每5分钟采集一次。该视频显示，由高增重日粮组公牛精液制备的胚胎，其发育进程存在延迟，且细胞质碎裂程度更为显著。