A 454 survey reveals the community composition and core microbiome of the common bed bug (Cimex lectularius) across an urban landscape

Elucidating the spatial dynamic and core constituents of the microbial communities found in association with arthropod hosts is of crucial importance for insects that may vector human or agricultural pathogens. The hematophagous Cimex lectularius (Hemiptera: Cimicidae), known as the human bed bug, has made a recent resurgence in North America, as well as worldwide, potentially owing to increased travel, climate change and resistance to insecticides. A comprehensive survey of the bed bug microbiome has not been performed to date, nor has an assessment of the spatial dynamics of its microbiome. Here we present a survey of internal and external bed bug microbial communities by amplifying the V4–V6 hypervariable region of the 16S rDNA gene region followed by 454 Titanium sequencing using 31 individuals from eight distinct collection locations obtained from residences in Cincinnati, OH. Across all samples, 97% of the microbial community is made up of two dominant OTUs, previously identified ...

阐明与节肢动物宿主共生的微生物群落的空间动态及核心组成，对于可能传播人类或农业病原体的昆虫而言具有至关重要的意义。吸血性温带臭虫（Cimex lectularius，半翅目：臭虫科）俗称人类床虱，近年来在北美乃至全球范围内再度泛滥，其种群回升趋势可能与出行频次增加、气候变化及杀虫剂抗药性有关。迄今为止，学界尚未对床虱的微生物组开展全面调查，也未对其微生物组的空间动态进行评估。本研究通过扩增16S rDNA基因的V4–V6高变区，并采用454 Titanium测序技术，对采自美国俄亥俄州辛辛那提市居民住宅的8个不同采样点的31只床虱个体的体表与体内微生物群落进行了调查分析。所有样本中，97%的微生物群落由两个此前已被鉴定的优势操作分类单元（OTU）构成……