Catalysis of MUF-triNAG by hen egg-white lysozyme (dataset formatted for analysis by interferENZY)

<strong>Main description</strong> This dataset depicts the catalysis of the fluorogenic substrate MUF-triNAG to the fluorescent substrate MUF by hen egg-white lysozyme, for fixed concentration of enzyme and variation of concentration of initial substrate. It was originally documented in <em>Phys. Chem. Chem. Phys., 2020, 22, 16143-16149</em> (https://doi.org/10.1039/D0CP02469D) and then used as a study case for the webserver interferENZY (a web-based tool for enzymatic assay validation and standardized kinetic analysis; visit https://interferenzy.i3s.up.pt for more information). To this end, it was converted to the format here presented: tab-separated *.txt input required for interferENZY analysis. <strong>Dataset organization</strong> Line 1: Tab-separated initial concentrations of substrate MUF-triNAG in micromolar (µM) concentration Line 2: Concentration of enzyme (0.25 µM) Line 3: Units of time Line 4: Units of concentration for substrate values and measurements Line 5: Dataset name Line 6 and downwards: Tab-separated column-pairs of the progress curves (time,Product) corresponding to the indicated values of initial concentrations of substrate in line 1 <strong>Contact information:</strong> Maria Filipa Pinto ([e-mail])<br> Pedro M. Martins ([e-mail]) i3S – Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal. Telephone number: [phone]## 主描述 本数据集记录了鸡卵清溶菌酶将产荧光底物MUF-triNAG催化为荧光产物MUF的酶促反应过程，实验中固定酶浓度，改变初始底物浓度。该数据集最初发表于《Phys. Chem. Chem. Phys., 2020, 22, 16143-16149》（DOI: 10.1039/D0CP02469D），后被用作在线工具interferENZY（一款用于酶活实验验证与标准化动力学分析的Web平台，详细信息请访问https://interferenzy.i3s.up.pt）的研究案例。为适配该工具的分析需求，本数据集已转换为当前格式：即interferENZY分析所需的制表符分隔*.txt输入文件。 ## 数据集组织结构 第1行：以制表符分隔的初始底物MUF-triNAG浓度（单位：微摩尔，µM） 第2行：酶浓度（0.25 µM） 第3行：时间单位 第4行：底物浓度值与检测结果的浓度单位 第5行：数据集名称 第6行及后续各行：对应第1行所列初始底物浓度的动力学进度曲线数据，以制表符分隔的列对形式呈现（时间，产物浓度） ## 联系方式 Maria Filipa Pinto（邮箱：[e-mail]） Pedro M. Martins（邮箱：[e-mail]） 葡萄牙波尔图大学健康研究与创新研究所（i3S – Instituto de Investigação e Inovação em Saúde），地址：Rua Alfredo Allen, 208, 4200-135 波尔图，葡萄牙 电话号码：[phone]