X-ray volume (xray3) registered to FIB-SEM of P7 mouse thymus (jrc_mus-thymus-1)

<br><b>Description</b>: X-ray volume (xray3) registered to FIB-SEM of P7 mouse thymus (jrc_mus-thymus-1)<b>X-ray ID</b>: xray3<b>Protocol</b>: After re-embedding in Durcupan, a 3D micro-CT scan of the entire block was performed using an XRadia Versa 510 X-ray microscope (Zeiss Microscopy). The tomogram facilitated precise selection of Regions of Interest (ROIs) and defined tissue boundaries. Multiple scans were conducted using different settings, with 4× and 20× objective lenses for overview and ROI selection, respectively. The sample block was then re-mounted on a 1 mm copper post for improved charge dissipation and trimmed to the ROI using an ultramicrotome. After each trimming step, X-ray tomograms were collected. Finally, a thin conductive layer was applied, consisting of a 10 nm gold layer followed by a 100 nm carbon layer.<b>Contributions</b>: X-ray microCT scans and trimming were performed by Zhiyuan Lu (HHMI/Janelia/EMSR). The sample collection through block trimming was supervised by Zhiheng Yu (HHMI/Janelia/EMSR). Regions of interest were selected by Christopher Bleck (HHMI/Janelia/EMSR), Wei-Ping Li (HHMI/Janelia/EMSR), Zhiyuan Lu, and Aubrey Weigel (HHMI/Janelia/CellMap Project Team). Data curation was handled by Rebecca Vorimo (HHMI/Janelia/CellMap Project Team) and Yurii Zubov (HHMI/Janelia/CellMap Project Team). The rest of the CellMap Project Team contributed to the overall development and review of the project. Aubrey Weigel provided oversight for the general project development.<b>X-ray voxel size (nm)</b>:<b>X-ray data dimensions (µm)</b>:<b>Dataset URL</b>: s3://janelia-cosem-datasets/jrc_mus-thymus-1/jrc_mus-thymus-1.zarr/recon-1/xray/xray-3/<b>Resulting, registered FIB-SEM volume</b>: https://doi.org/10.25378/janelia.24085287<br><br><b>Visualization website</b>: https://openorganelle.janelia.org/datasets/jrc_mus-thymus-1

<br><b>描述</b>: 配准至P7天龄小鼠胸腺聚焦离子束扫描电子显微镜（FIB-SEM）数据集的X射线体数据集（xray3）（样本编号：jrc_mus-thymus-1）<br><b>X射线编号</b>: xray3<br><b>实验方案</b>: 将样本块经Durcupan树脂重新包埋后，使用XRadia Versa 510型X射线显微镜（Zeiss Microscopy）对整个样本块开展三维显微CT扫描。该断层扫描数据可精准选取感兴趣区域（Regions of Interest, ROIs）并明确组织边界。实验采用多组不同参数设置完成多轮扫描，分别使用4×和20×物镜完成概览扫描与感兴趣区域选取。随后将样本块重新安装至1 mm铜柱以优化电荷耗散，并使用超薄切片机将样本修切至目标感兴趣区域。每完成一次修切步骤，即采集一轮X射线断层扫描数据。最终在样本表面沉积薄层导电膜：先沉积10 nm金层，再沉积100 nm碳层。<br><b>贡献者</b>: X射线显微CT扫描与样本修切工作由卢致远（Zhiyuan Lu, HHMI/Janelia/EMSR）完成。样本修切阶段的样本采集工作由喻智恒（Zhiheng Yu, HHMI/Janelia/EMSR）监督。感兴趣区域的选取工作由Christopher Bleck（HHMI/Janelia/EMSR）、李卫平（Wei-Ping Li, HHMI/Janelia/EMSR）、卢致远以及Aubrey Weigel（HHMI/Janelia/细胞图谱项目组（CellMap Project Team））共同完成。数据整理工作由Rebecca Vorimo（HHMI/Janelia/细胞图谱项目组（CellMap Project Team））与Yurii Zubov（HHMI/Janelia/细胞图谱项目组（CellMap Project Team））负责。其余细胞图谱项目组人员参与了项目的整体开发与审核工作。Aubrey Weigel负责项目整体开发的监督工作。<br><b>X射线体素尺寸（nm）</b>:<br><b>X射线数据维度（µm）</b>:<br><b>数据集URL</b>: s3://janelia-cosem-datasets/jrc_mus-thymus-1/jrc_mus-thymus-1.zarr/recon-1/xray/xray-3/<br><b>最终配准的FIB-SEM体数据集</b>: https://doi.org/10.25378/janelia.24085287<br><br><b>可视化网站</b>: https://openorganelle.janelia.org/datasets/jrc_mus-thymus-1