Supplementary Material for: Using Component-Resolved Diagnosis to Characterize the Sensitization to Specific Cat and Dog Allergens in Patients with Allergic Respiratory Diseases in Catalonia, Spain

Introduction: Sensitization to cat and dog allergens is common in patients with allergic respiratory diseases. The study objective was to determine the prevalence of immunoglobulin E (IgE) sensitization to specific cat and dog allergens using component-resolved diagnosis (CRD) in patients with allergic respiratory diseases plus cat and/or dog sensitization. Methods: We included 87 patients aged 8–62 years, diagnosed with allergic asthma and/or rhinitis plus cat and/or dog sensitization, and attended at the allergy section of a tertiary hospital in Badalona (Catalonia, Spain). We used CRD to determine IgE sensitization to specific cat/dog allergens and skin prick tests (SPTs) to determine differences between diagnostic test results. Results: Patients were monosensitized to cats (20.7%) or dogs (3.4%) or sensitized to both (75.9%). The highest positive allergen rates were for Fel d 1 (91.7%) and Fel d 4 (41%) in patients sensitized to cat allergens and for Can f 5 (80%) and Can f 1 (70%) in those sensitized to dog allergens. CRD and SPT results differed somewhat: 16.1% and 27.6% of patients CRD positive for cat or dog sensitization, respectively, were SPT negative, and 6.9% SPT positive for dog sensitization were CRD negative. Few statistically significant relationships were found between any allergen components and any respiratory disease characteristic or contact with furry animals. Conclusions: CRD may be used to determine the prevalence of IgE sensitization to specific cat and dog allergens in patients with allergic respiratory diseases plus cat and/or dog sensitization. As SPT may not correctly identify all patients sensitized to cats and dogs, our results support the use of CRD.

引言：猫、犬过敏原致敏在过敏性呼吸道疾病患者中较为普遍。本研究旨在借助组分诊断（component-resolved diagnosis, CRD），明确合并猫和/或犬致敏的过敏性呼吸道疾病患者体内，免疫球蛋白E（immunoglobulin E, IgE）针对特定猫、犬过敏原的致敏率。
方法：本研究纳入87例年龄介于8~62岁的患者，均确诊为过敏性哮喘和/或过敏性鼻炎，且合并猫和/或犬致敏，就诊于西班牙加泰罗尼亚巴达洛纳某三级医院的变态反应科。本研究采用CRD检测患者针对特定猫/犬过敏原的IgE致敏情况，并通过皮肤点刺试验（skin prick tests, SPTs）对比两种诊断方法的检测结果差异。
结果：患者中，仅对猫致敏者占20.7%，仅对犬致敏者占3.4%，同时对两类过敏原均致敏者占75.9%。在猫过敏原致敏患者中，阳性检出率最高的过敏原组分为Fel d 1（91.7%）与Fel d 4（41%）；在犬过敏原致敏患者中，阳性检出率最高的组分为Can f 5（80%）与Can f 1（70%）。CRD与SPTs的检测结果存在一定差异：分别有16.1%、27.6%的猫、犬过敏原CRD阳性患者SPT结果为阴性；另有6.9%的犬致敏SPT阳性患者CRD结果为阴性。未发现任意过敏原组分与呼吸道疾病特征或毛茸动物接触史之间存在显著统计学关联。
结论：针对合并猫和/或犬致敏的过敏性呼吸道疾病患者，CRD可用于明确其针对特定猫、犬过敏原的IgE致敏率。鉴于SPTs无法准确识别所有猫、犬致敏患者，本研究结果支持采用CRD开展相关检测。