Identification of host factors that bind to the 5? end of the MERS-CoV RNA genome: supplementary data

Aim: The aim of this study was to identify host factors that interact with the 5? end of the MERS-CoV RNA genome. Materials & methods: RNA affinity chromatography followed by mass spectrometry analysis was used to identify the binding of host factors in Vero E6 cells. Results: A total of 59 host factors that bound the MERS-CoV RNA genome in non-infected Vero E6 cells were identified. Most of the identified cellular proteins were previously reported to interact with the genome of other RNA viruses.We validated our mass spectrometry results using western blotting. Conclusion: These data enhance our knowledge about the RNA–host interactions of coronaviruses, which could serve as targets for developing antiviral therapeutics against MERS-CoV.研究目的：本研究旨在鉴定可与中东呼吸综合征冠状病毒（Middle East Respiratory Syndrome Coronavirus, MERS-CoV）RNA基因组5'端相互作用的宿主因子。材料与方法：采用RNA亲和层析联合质谱分析的方法，对Vero E6细胞内的宿主因子结合情况进行鉴定。研究结果：在未感染的Vero E6细胞中，共鉴定出59种可结合MERS-CoV RNA基因组的宿主因子。绝大多数已鉴定的细胞蛋白此前已有研究报道其可与其他RNA病毒的基因组相互作用。我们通过蛋白质印迹（Western Blotting）对质谱分析结果进行了验证。研究结论：本研究数据深化了我们对冠状病毒RNA-宿主相互作用机制的认知，上述宿主因子可作为开发抗MERS-CoV抗病毒治疗药物的潜在靶点。