Dataset of SARS-CoV-2 wastewater data from Älvkarleby, Sweden

This is a metadata record for a continuously updated dataset of SARS-CoV-2 RNA data in wastewater in Älvkarleby, Sweden. The dataset is part of a research study led associate professor Anna J. Székely (SLU, Swedish University of Agricultural Sciences) and her research groups in collaboration with Uppsala Vatten. The research group is part of the Environmental Virus Profiling Research Area of the SciLifeLab National COVID-19 Research Program.The viral content is concentrated according to the protocol from Ahmed et al. https://doi.org/10.1016/j.scitotenv.2020.139960according to the modified electronegative filtration method. Viral RNA is extracted and SARS-CoV-2 gene copy nr quantified using R-qPCR. Recovery efficiency is measured using Bovine coronavirus (BCoV) as a virus surrogate. Pepper mild mottle virus (PPMoV) is used to correct for variations in water size and flow. For more information see https://www.covid19dataportal.se/data_types/environment/wastewater/#uppsalaThe dataset for Älvkarleby started week 14 2021.The dataset is available as part of the Environmental Virus Profiling data section. The amount of SARS-CoV-2 virus in wastewater across Sweden" https://www.covid19dataportal.se/data_types/environment/wastewater/#uppsala on the Swedish COVID-19 Data Portal (https://covid19dataportal.se). The dataset is also available as part of the CRUSH Covid project https://crush-covid.shinyapps.io/crush_covid/Funded by SciLifeLab/KAW National COVID-19 Research Program https://www.scilifelab.se/covid-19/national-program/本文件为瑞典埃尔夫卡勒比（Älvkarleby）地区污水处理厂新冠病毒（SARS-CoV-2）RNA监测的持续更新数据集的元数据记录。本数据集由瑞典农业科学大学（Swedish University of Agricultural Sciences, SLU）安娜·J·塞克利（Anna J. Székely）副教授带领其研究团队，与乌普萨拉水务（Uppsala Vatten）合作开展的研究项目的组成部分。该研究团队隶属于瑞典生命科学实验室（SciLifeLab）国家新冠疫情研究计划的环境病毒谱分析研究领域。病毒核酸浓缩流程参照Ahmed等人提出的改良负电过滤法方案（https://doi.org/10.1016/j.scitotenv.2020.139960）。提取病毒RNA后，采用反转录实时定量聚合酶链式反应（R-qPCR）对新冠病毒（SARS-CoV-2）的基因拷贝数进行定量检测。以牛冠状病毒（BCoV）作为病毒替代物，对检测过程的回收效率进行测定；以辣椒轻斑驳病毒（PPMoV）作为内参，校正水样体积与流速带来的检测偏差。更多详细信息可参阅：https://www.covid19dataportal.se/data_types/environment/wastewater/#uppsala。埃尔夫卡勒比地区的该数据集监测起始于2021年第14周。本数据集隶属于环境病毒谱分析数据集板块。瑞典全国范围内污水处理厂中的新冠病毒（SARS-CoV-2）载量数据可通过瑞典新冠疫情数据门户（https://covid19dataportal.se）的相关页面（https://www.covid19dataportal.se/data_types/environment/wastewater/#uppsala）获取。本数据集同时也可在CRUSH新冠项目平台（https://crush-covid.shinyapps.io/crush_covid/）获取。本研究由瑞典生命科学实验室与KAW联合资助的国家新冠疫情研究计划（https://www.scilifelab.se/covid-19/national-program/）资助。