Comparison of experimental and calculated mean distances for the fluorophore pairs.

Cα-Cα distances are determined from the crystal structure (pdb: 1GO3). FRET distances are derived from the data collected at 25°C, calculated from the simulated distance distributions, taking the 1/r6 dependency of the transfer efficiency into account.Values given in brackets are the maxima of the distance distributions. Errors given for the FRET distances represent the standard deviations, those given for the calculated distances from the simulations represent the width of the distance distribution at half maximum.