The circadian transcriptome of liver and muscle in aging mice treated with 3dA

The decline of behavioral and metabolic rhythms with aging underscores the critical role of circadian regulation in health and longevity. Restoring circadian rhythms has recently emerged as a promising strategy to combat aging-associated disorders. We demonstrate that 3-deoxyadenosine (3dA) enhances circadian rhythm amplitude of cells and consolidates daily rhythmicity in behavior and energy metabolism in a time-of-day-dependent manner. Timed treatment with 3dA significantly mitigates pathological aging processes. To investigate the mechanism of time-restricted 3dA administration on aging, we conducted time-course RNA landscape analysis of aged mice, with or without 3dA treatment for 3 months. Considering the observed reduction of age-related markers in the liver of mice and the enhancement of muscle function in mice during our experiments, we primarily analyzed the circadian transcriptome of these two tissues. Results suggests that 3dA reprograms the rhythmic RNA landscape towards a synchrony and robustness clock in endocrine oscillation