Gene expression profiles of endothelium, microglia and oligodendrocytes in hippocampus of post-stroke depression rat at single cell resolution

Post-stroke depression (PSD) is a common but severe mental complication after stroke. However, the cellular and molecular understanding of PSD is still yet to be illustrated. In current study, we prepared PSD rat model (MD) via unilateral middle cerebral artery occlusion (MCAO) and chronic stress stimulation (DEPR), and isolated hippocampal tissues for single cell sequencing of 10x Genomics Chromium. This study characterized the single cell expression profile of hippocampal PSD, and unmask the differential expressed genes of endothelium, microglia and oligodendrocytes, emphasizing the crosstalk among them to provide theoretical basis for the in-depth mechanism research and drug therapy of PSD. Twenty male adult Sprague Dawley rats aged with 10-12 months (Weight 250-300 g) were randomly divided into two groups (n = 10). These two group were subject to the unilateral intraluminal monofilament in middle cerebral artery for MCAO. One group is then received chronic stress stimulation for depression. Hippocampus tissues in each group (n = 5) were used for single cell sequencing using 10x Genomics platform.