S1 Table. Primer sequences and Accession # utilized for real time PCR analysis of immune genes

All primer sequences were designed to span exon-exon junctions, to minimize the potential of amplifying genomic DNA, using Primer3 software with sequences obtained from GenBank (http://www.ncbi.nlm.nih.gov/). All primer pairs displayed melting curves with a single peak, indicative of a pure, single amplicon, confirmed the specificity of the primers.

所有引物序列均设计为跨外显子-外显子交界区域，以尽可能降低扩增基因组DNA的风险，采用Primer3软件，以GenBank（http://www.ncbi.nlm.nih.gov/）中获取的序列为模板完成设计。所有引物对均呈现单峰熔解曲线，表明仅扩增得到单一纯净的产物，从而证实了引物的特异性。