Identification and analysis of oil candidate genes reveals the molecular basis of cottonseed oil accumulation in Gossypium hirsutum L.

<b>Table S1. </b><b> </b>ANOVA and broad-sense heritability analysis for cottonseed oil content.<b></b> <b>Table S2. </b>QTLs related to cottonseed oil content identified in eight environments. <b>Table S3. </b>832 genes in the five stable QTL regions. <b>Table S4.</b> KEGG pathway enrichment analysis of 832 genes around stable QTLs. <b>Table S5.</b> Genes list identified by BLAST with <i>Arabidopsis thaliana</i> oil-related genes. <b>Table S6. </b>The expression level (FPKM values) of candidate oil genes during developing ovules of <i>G. hirsutum</i> L.<b></b> <b>Table S7. </b>Four transcription factors related to cottonseed lipid content. <b>Table S8. </b>The microRNA-target gene regulatory network of <i>G. hirsutum</i> L. <b>Table S9.</b> The designed primers of genes encoding the key enzymes involved in lipid biosynthesis for qRT-PCR.

补充表S1. 棉籽油含量的方差分析（ANOVA）与广义遗传力（broad-sense heritability）分析。
补充表S2. 八个环境下鉴定得到的与棉籽油含量相关的数量性状基因座（QTL）。
补充表S3. 五个稳定数量性状基因座区域内的832个基因。
补充表S4. 稳定数量性状基因座侧翼832个基因的京都基因与基因组百科全书（KEGG）通路富集分析。
补充表S5. 以拟南芥（Arabidopsis thaliana）油脂相关基因为参考，通过基本局部比对搜索工具（BLAST）鉴定得到的基因列表。
补充表S6. 陆地棉（G. hirsutum L.）发育胚珠中候选油脂基因的表达水平（FPKM，每百万映射片段中每千碱基转录本的片段数）值。
补充表S7. 四个与棉籽脂质含量相关的转录因子（transcription factors）。
补充表S8. 陆地棉（G. hirsutum L.）的微小RNA-靶基因调控网络（microRNA-target gene regulatory network）。
补充表S9. 脂质生物合成关键酶编码基因的实时定量聚合酶链反应（qRT-PCR）专用设计引物。