molecules 2 in A Heterolepidoderma and Halichaetoderma gen. nov. (Gastrotricha: Chaetonotidae) riddle: integrative taxonomy and phylogeny of six new freshwater species from Central Europe

molecules 2 ITS analysed the of Characterization. 2 Table TaxonTotalGC contentLength of helixNumber of unpaired bases inNo. ofNo. of GUΔ G (37 ° C,length(%)I II III IVCentral loopTerminal loop of helix ITerminal loop of helix IITerminal loop of helix IIITerminal loop of helix IVBulge (s) of helix IIIBulges of helix IVbulges in helix IVpairingskcal / mol)Heterolepidoderma kolickae17036.4773139563737984825‒ 34.40Heterolepidoderma striatum14242.96525345226376721336‒ 29.10Heterolepidoderma macrops16934.3212294059296747610311‒ 34.40Halichaetoderma fluviatile20640.7812275578344777162156‒ 42.00Halichaetoderma rivale23139.3925313211231774764178‒ 45.90Minimum14234.3252532522637472825‒ 45.90Maximum23142.962531551123777981641711‒ 29.10Arithmetic mean183.638.7812.228.640.071.431.44.67.06.07.26.818.64.07.2‒ 37.20Standard deviation34.923.437.79 2.61 9.03 24.804.281.820.002.120.455.4013.462.002.396.71### 基于分子标记2 内转录间隔区（Internal Transcribed Spacer，ITS）的表征分析数据集。表2 | 分类群 | 总GC含量(%) | 螺旋长度 | 螺旋I未配对碱基数 | 螺旋II未配对碱基数 | 螺旋III未配对碱基数 | 螺旋IV未配对碱基数 | 中央环数目 | 螺旋I末端环数目 | 螺旋II末端环数目 | 螺旋III末端环数目 | 螺旋IV末端环数目 | 螺旋III凸起数目 | 螺旋IV凸起数目 | 螺旋IV内部凸起数目 | GU配对数目 | ΔG(37℃，kcal/mol) | | ---- | ---- | ---- | ---- | ---- | ---- | ---- | ---- | ---- | ---- | ---- | ---- | ---- | ---- | ---- | ---- | ---- | | 科利克异毛皮虫（Heterolepidoderma kolickae） | 36.47 | 73 | 13 | 9 | 5 | 6 | 3 | 7 | 3 | 7 | 9 | 8 | 4 | 8 | 25 | -34.40 | | 条纹异毛皮虫（Heterolepidoderma striatum） | 42.96 | 65 | 25 | 34 | 5 | 2 | 2 | 6 | 3 | 7 | 6 | 7 | 2 | 13 | 36 | -29.10 | | 大眼异毛皮虫（Heterolepidoderma macrops） | 34.32 | 122 | 9 | 40 | 5 | 9 | 2 | 9 | 6 | 7 | 4 | 7 | 6 | 10 | 31 | -34.40 | | 河生盐毛皮虫（Halichaetoderma fluviatile） | 40.78 | 122 | 7 | 55 | 7 | 8 | 3 | 4 | 4 | 7 | 7 | 7 | 1 | 6 | 21 | -42.00 | | 溪生盐毛皮虫（Halichaetoderma rivale） | 39.39 | 253 | 13 | 21 | 12 | 3 | 1 | 7 | 7 | 4 | 7 | 6 | 4 | 17 | 8 | -45.90 | | 最小值 | 34.32 | 52 | 5 | 32 | 5 | 2 | 2 | 6 | 3 | 7 | 4 | 7 | 2 | 8 | 25 | -45.90 | | 最大值 | 42.96 | 253 | 15 | 55 | 11 | 2 | 3 | 7 | 7 | 7 | 9 | 8 | 1 | 6 | 41 | -29.10 | | 算术均值 | 38.78 | 122.2 | 8.6 | 4.0 | 7.1 | 4.3 | 1.4 | 3.1 | 4.6 | 7.0 | 6.0 | 7.2 | 6.8 | 18.6 | 4.0 | -37.20 | | 标准差 | 34.92 | 3.43 | 7.79 | 2.61 | 9.03 | 24.80 | 4.28 | 1.82 | 0.00 | 2.12 | 0.45 | 5.40 | 13.46 | 2.00 | 2.39 | 6.71 |