Carcass size, not source or taxon, dictates breeding performance and carcass use in burying beetle

This repository contains the data and R code for analyzing the breeding outcomes, carcass use, and larval growth of the burying beetle as well as the nutritional composition of carcass tissue. (Information below is also provided in the README file.) 01_Data_Raw: This folder contains three original datasheets recorded during data collection. File "Breeding_Data_All.xls": This file contains the raw data on carcass attributes, parent sizes, breeding outcomes, and carcass use from the breeding experiments. Each row represents an observation from one breeding pair. File "Nutrition_Data.xls": This file contains the raw data on the tissue nutrient content of lab and wild carcasses from the nutritional composition analysis. Each row represents an observation from one carcass tissue sample. File "Larval_Growth_Data.xls": This file contains the raw data on the larval weight from the feeding experiments. Each row represents an observation from one larva. 02_R_Code: This folder contains the R scripts for data analyses and visualization. File "01_Data_Cleaning.R": This script cleans and organizes the raw datasheets in the folder "01_Data_Raw" and saves the cleaned datasheets in the folder "03_Outputs > Data_Clean" for data analyses and visualization. File "02_Models_by_Carcass_Source.R": The script analyzes the relationships between carcass size vs. breeding outcomes and carcass use efficiency on lab and wild carcasses. File "03_Models_by_Carcass_Taxon.R": The script analyzes the breeding outcomes on wild carcasses (mammals, birds, and reptiles). File "04_Models_Nutrition_and_Larval_Growth.R": This script analyzes the tissue nutrient content of lab and wild carcasses as well as the larval growth on these carcasses. File "05_Figures.R": This script generates the figures in the study. 03_Outputs: This folder contains a subfolder "Data_Clean", which contains three cleaned datasheets used for data analyses and visualization. File "Breeding_Data_Clean.csv": This file contains cleaned data on carcass attributes, parent sizes, breeding outcomes, and carcass use from the breeding experiments. Each row represents an observation from one breeding pair. File "Nutrition_Data_Clean.csv": This file contains the cleaned data on the tissue nutrient content of lab and wild carcasses from the nutritional composition analysis. Each row represents an observation from one carcass tissue sample. File "Larval_Growth_Clean.csv": This file contains the cleaned data on the larval weight from the feeding experiments. Each row represents an observation from one larva. Column descriptions File "Breeding_Data_Clean.csv": 1. date: The starting date of the breeding experiments. 2. carcass_sp_Chinese: The Chinese name of the carcass animal. 3. carcass_type: The source of the carcass (lab or wild). 4. carcass_taxon: The taxon of the carcass (mammal, bird, or reptile). 5. carcass_weight: The initial weight of the carcass (g). 6. parent_generation: The generation number of the breeding parents. 7. pair_id: The ID of the breeding pair. 8. generation_pair_id: The combined ID of the generation number and breeding pair. 9. male_size: The pronotum width of the male parent (mm). 10. female_size: The pronotum width of the female parent (mm). 11. clutch_size: The number of eggs laid by the female. 12. n_larvae: The number of larvae. 13. total_larval_mass: The total weight of the larvae. 14. carcass_weight_loss: The difference between the initial carcass weight and the carcass weight at the end of the breeding experiments. 15. breeding_success: Whether there was at least one larva in the breeding container. 16. prop_eggs_developed: The proportion of eggs that developed as larvae, calculated as the number of larvae divided by clutch size. 17. average_larval_mass: The average weight of each larva, calculated as the total larval weight divided by the number of larvae. 18. larval_density: The density of larvae on the carcass, calculated as the number of larvae divided by carcass weight. 19. prop_carcass_used: The proportion of carcass tissue used by the larvae, calculated as carcass weight loss divided by the initial carcass weight. File "Nutrition_Data_Clean.csv": 1. block_id: The ID of the analysis. 2. carcass_id: The ID of the carcass. 3. carcass_type: The source of the carcass (lab or wild). 4. carcass_taxon: The taxon of the carcass (mammal, bird, or reptile). 5. tissue_type: The type of the carcass tissue sampled (muscle or viscera). 6. tissue_replication: The replication number of the tissue sample from each carcass. 7. wet_mass_g: The wet weight of the tissue sample (g). 8. water_mass_g: The water weight of the tissue sample (g). 9. dry_mass_g: The dry weight of the tissue sample (g). 10. protein_mass_g: The protein weight of the tissue sample (g). 11. fat_mass_g: The fat weight of the tissue sample (g). 12. total_mass_g: The total water, protein, and fat weight of the tissue sample (g). 13. prop_protein: The proportion of protein content in the tissue sample, calculated as the protein weight divided by the total weight. 14. prop_fat: The proportion of protein content in the tissue sample, calculated as the fat weight divided by the total weight. File "Larval_Growth_Clean.csv": 1. block_id: The ID of the experimental block (two rounds of feeding experiments were conducted). 2. carcass_id: The ID of the carcass (nested within the experimental block). 3. carcass_type: The source of the carcass (lab or wild). 4. carcass_taxon: The taxon of the carcass (mammal, bird, or reptile). 5. tissue_type: The type of the carcass tissue sampled (muscle or viscera). 6. larva_replication: The larva replication number (nested within each tissue type and carcass ID). 7. tissue_mass_g: The weight of the carcass tissue fed to the larva. 8. family_id: The family ID of the parents of the larva. 9. success: Whether the larva survived. 10. initial_larval_mass_g: The larval weight at the start of the experiment (g). 11. end_larval_mass_g: The larval weight at the end of the experiment (g). 12. larval_weight_gain_g: The difference between the initial larval weight and the end larval weight (g). 13. mean_prop_protein: The average protein content of the three tissue samples from each carcass. 14. mean_prop_fat: The average fat content of the three tissue samples from each carcass.本仓库包含用于分析葬甲（burying beetle）繁殖结果、尸体利用情况、幼虫生长状况，以及尸体组织营养组成的相关数据与R代码。下文说明亦见README文件。 01_Data_Raw：本文件夹包含数据采集过程中记录的三份原始数据表。 "Breeding_Data_All.xls"：该文件包含繁殖实验中关于尸体属性、亲本体型、繁殖结果及尸体利用情况的原始数据。每一行代表一个繁殖对的观测数据。 "Nutrition_Data.xls"：该文件包含营养组成分析中，实验室来源与野生来源尸体的组织营养含量原始数据。每一行代表一份尸体组织样本的观测数据。 "Larval_Growth_Data.xls"：该文件包含取食实验中幼虫体重的原始数据。每一行代表单头幼虫的观测数据。 02_R_Code：本文件夹包含用于数据分析与可视化的R脚本。 "01_Data_Cleaning.R"：该脚本用于清洗并整理"01_Data_Raw"文件夹内的原始数据表，并将清洗后的数据表保存至"03_Outputs > Data_Clean"文件夹，以供后续数据分析与可视化使用。 "02_Models_by_Carcass_Source.R"：该脚本用于分析尸体大小与繁殖结果、实验室及野生尸体的资源利用效率之间的关联关系。 "03_Models_by_Carcass_Taxon.R"：该脚本用于分析不同分类群（哺乳类、鸟类、爬行类）野生尸体对应的繁殖结果。 "04_Models_Nutrition_and_Larval_Growth.R"：该脚本用于分析实验室与野生来源尸体的组织营养组成，以及取食上述尸体的幼虫生长状况。 "05_Figures.R"：该脚本用于生成本研究中的所有图表。 03_Outputs：本文件夹包含子文件夹"Data_Clean"，其中包含三份用于数据分析与可视化的清洗后数据表。 "Breeding_Data_Clean.csv"：该文件包含清洗后的繁殖实验相关数据，涵盖尸体属性、亲本体型、繁殖结果及尸体利用情况。每一行代表一个繁殖对的观测数据。 "Nutrition_Data_Clean.csv"：该文件包含清洗后的营养组成分析数据，涵盖实验室与野生来源尸体的组织营养含量。每一行代表一份尸体组织样本的观测数据。 "Larval_Growth_Clean.csv"：该文件包含清洗后的取食实验幼虫体重数据。每一行代表单头幼虫的观测数据。 ### 字段说明 #### 「Breeding_Data_Clean.csv」 1. date：繁殖实验的起始日期。 2. carcass_sp_Chinese：尸体来源动物的中文名称。 3. carcass_type：尸体的来源类型（实验室来源或野生来源）。 4. carcass_taxon：尸体的分类类群（哺乳类、鸟类或爬行类）。 5. carcass_weight：尸体初始重量（单位：克）。 6. parent_generation：繁殖亲本的世代数。 7. pair_id：繁殖对的编号。 8. generation_pair_id：世代编号与繁殖对编号的组合ID。 9. male_size：雄性亲本的前胸背板宽度（单位：毫米）。 10. female_size：雌性亲本的前胸背板宽度（单位：毫米）。 11. clutch_size：雌性产卵的数量。 12. n_larvae：幼虫的数量。 13. total_larval_mass：幼虫总重量。 14. carcass_weight_loss：繁殖实验结束时尸体重量与初始尸体重量的差值。 15. breeding_success：繁殖容器中是否存在至少一头幼虫（即繁殖是否成功）。 16. prop_eggs_developed：卵发育为幼虫的比例，计算方式为幼虫数除以产卵总数。 17. average_larval_mass：单头幼虫的平均体重，计算方式为幼虫总重量除以幼虫数量。 18. larval_density：尸体上的幼虫密度，计算方式为幼虫数量除以尸体初始重量。 19. prop_carcass_used：幼虫利用的尸体组织比例，计算方式为尸体重量损失除以尸体初始重量。 #### 「Nutrition_Data_Clean.csv」 1. block_id：分析批次ID。 2. carcass_id：尸体ID。 3. carcass_type：尸体的来源类型（实验室来源或野生来源）。 4. carcass_taxon：尸体的分类类群（哺乳类、鸟类或爬行类）。 5. tissue_type：采样的尸体组织类型（肌肉或内脏）。 6. tissue_replication：单具尸体的组织样本重复编号。 7. wet_mass_g：组织样本的湿重（单位：克）。 8. water_mass_g：组织样本的水分重量（单位：克）。 9. dry_mass_g：组织样本的干重（单位：克）。 10. protein_mass_g：组织样本的蛋白质重量（单位：克）。 11. fat_mass_g：组织样本的脂肪重量（单位：克）。 12. total_mass_g：组织样本中水、蛋白质与脂肪的总重量（单位：克）。 13. prop_protein：组织样本中蛋白质的含量占比，计算方式为蛋白质重量除以总重量。 14. prop_fat：组织样本中蛋白质的含量占比，计算方式为脂肪重量除以总重量。 #### 「Larval_Growth_Clean.csv」 1. block_id：实验批次ID（本研究共开展两轮取食实验）。 2. carcass_id：尸体ID（嵌套于实验批次内）。 3. carcass_type：尸体的来源类型（实验室来源或野生来源）。 4. carcass_taxon：尸体的分类类群（哺乳类、鸟类或爬行类）。 5. tissue_type：采样的尸体组织类型（肌肉或内脏）。 6. larva_replication：单组织类型与尸体ID内的幼虫重复编号。 7. tissue_mass_g：投喂给幼虫的尸体组织重量（单位：克）。 8. family_id：幼虫亲本的家系ID。 9. success：幼虫是否存活。 10. initial_larval_mass_g：实验开始时的幼虫体重（单位：克）。 11. end_larval_mass_g：实验结束时的幼虫体重（单位：克）。 12. larval_weight_gain_g：实验结束时幼虫体重与实验开始时体重的差值（单位：克）。 13. mean_prop_protein：单具尸体的三份组织样本的平均蛋白质含量占比。 14. mean_prop_fat：单具尸体的三份组织样本的平均脂肪含量占比。