Electrophysiological development and functional plasticity in dissociated human cerebral organoids across multiple cell lines - Raw MEA data (2 of 4)

Microelectrode arrays (MEAs) are increasingly used to profile the development of synchronised activity in neural organoids, yet no organoid study has investigated the consistency of electrophysiological development across cell lines. Here, we used dissociated neural organoids derived from six cell lines on MEAs to characterise functional synapse development using multiple parameters across time. The dissociated organoids had increasing functional connectivity and network activity over time across all cell lines and plasticity in response to synaptic-like stimulation. Like the organoids they were derived from, dissociated organoid cultures contained a diverse mixture of cell types. Variability in activity parameters was associated with differences in cell type composition and regional identity, which in turn were affected by donor cell line and batch effects. These results demonstrate that dissociated cerebral organoids can generate functional neurons, akin to primary neuronal cultures from brain tissue, providing a scalable model for studies of neurodevelopment and synaptic function.The raw and well average data deposited here consists of 5-minute recordings of dissociated organoids on MEAs over time, as well as a chemical LTP plasticity treatment.

微电极阵列（Microelectrode Arrays, MEAs）正愈发广泛地被用于解析神经类器官的同步活动发育进程，但目前尚无类器官相关研究探讨不同细胞系间电生理发育的一致性。本研究采用源自6株细胞系的解离型神经类器官，依托微电极阵列，通过多维度时间序列参数对功能性突触发育进行表征。所有细胞系的解离型类器官均随培养时长增加，呈现出功能性连接与网络活动的逐步增强，同时可对类突触刺激产生可塑性反应。与其来源的原始类器官一致，解离型类器官培养物同样包含多种细胞类型的混合群体。活动参数的变异与细胞类型组成及区域特性的差异相关，而这些差异又受供体细胞系与批次效应的影响。本研究结果表明，解离型大脑类器官可产生功能性神经元，其特性与脑组织来源的原代神经元培养物相似，可为神经发育与突触功能研究提供可规模化应用的实验模型。本数据集所存档的原始数据与各培养孔平均数据，包含了随时间序列采集的解离型类器官在微电极阵列上的5分钟时长记录，以及化学诱导长时程增强（Long-term Potentiation, LTP）可塑性处理的相关数据集。