Growth curve of algae and betaine content determination

Betaine and carnitine standard/Sample solutions were derivatised with a mixture of 2-Bromo-2'-acetonaphthone and 18-crown-6 in N-methyl -2 pyrrolidone in presence of KH₂PO₄ (0.1 M) at 80^◦C (4 h). After filtering, the solution was eluted with 22 mM Choline chloride in 10% H₂O/90% ACN at a flow rate of 1.9 ml/min (Injection 5 µL) at 40^◦C. Betaine content in microalgae biomass was determined using calibration curve of standard betaine.<br>

甜菜碱（Betaine）与肉碱（carnitine）的标准溶液及样品溶液，在0.1 mol/L磷酸二氢钾（KH₂PO₄）存在条件下，于80℃中采用2-溴-2'-乙酰萘（2-Bromo-2'-acetonaphthone）与18-冠-6（18-crown-6）的混合液在N-甲基-2-吡咯烷酮（N-methyl-2-pyrrolidone）中进行衍生化反应，反应时长为4小时。过滤完成后，将所得溶液以1.9 mL/min的流速进行洗脱，洗脱液为含22 mM氯化胆碱（Choline chloride）的10%水/90%乙腈（ACN）混合溶剂，柱温设置为40℃，进样量为5 μL。采用甜菜碱标准品绘制校准曲线，对微藻生物质中的甜菜碱含量进行定量测定。