Metagenome assembled genomes co_2-Bin_19_1.fa

Co-assemblies were binned with CONCOCT within anvi’o v5.1 by clustering scaffolds 2500 bp or longer into metagenome assembled genomes (MAGs) and manually refining them within the anvi’o platform. Furthermore, dRep v2.3.2 was used to dereplicate MAGs and obtain representative genomes with flags ‘-comp 50 -con 10’ and default values.

本研究借助anvi’o v5.1平台中的CONCOCT工具对共组装产物进行分箱：具体操作为将长度不低于2500 bp的支架序列（scaffolds）聚类为宏基因组组装基因组（metagenome assembled genomes, MAGs），并在该平台内完成人工精修。此外，本研究使用dRep v2.3.2工具对所得MAGs进行去冗余处理，并通过参数‘-comp 50 -con 10’及默认参数筛选获得代表性基因组。