Improved methane mitigation potential and modulated methane cycling microbial communities in arable soil by compost addition

The global atmospheric concentration of the potent greenhouse gas methane (CH4) is rising rapidly, and agriculture is responsible for 30-50% of the yearly CH4 emissions. To limit its global warming effects, strong and sustained reductions are needed. Sustainable agricultural management strategies, as the use of organic amendments like compost, have previously proven to have a potent CH4 mitigation effect in laboratory experiments. Here we investigated, using an extensive field study, the effect of organic amendments on the CH4 mitigation potential of agricultural soils and the CH4 cycling microbial communities. Organic-amended soils had higher potential CH4 uptake rates and an improved potential to oxidize CH4 to sub-atmospheric concentrations. Also, we showed for the first time that the methanotrophic and methanogenic microbial communities of arable soils were unequivocally altered after organic amendment application by increasing in size while getting less diverse. Compost-amended soi..., , # Improved methane mitigation potential and modulated methane cycling microbial communities in arable soil by compost addition

Dataset DOI: [10.5061/dryad.dz08kps8h](10.5061/dryad.dz08kps8h)

## Description of the data and file structure

To assess the effect of organic fertilization on the methane uptake potential and methane cycling microbial communities of agricultural soils, an extensive field screening was performed.

To this end, soil samples (0-15cm depth) were collected at 6 sites in the Netherlands, Belgium and Switzerland. The following physicochemical properties were determined: Moisture content, organic matter content, pH, NO3+NO2-N-, NH4-N, Al-, Co-, Cu-, Fe-, K-, Mg-, Mn-, Mo-, Na-, Ni-, P-, S-, and Zn-content. Also, the potential methane uptake rates and lag phases were determined in incubations with 10 or 10.000 ppmv CH4 in the headspace, and the capacity to oxidize to sub-atmospheric CH4 concentrations. Furthermore, the gene copy numbers of the bacterial 16S rRNA, *pmo...,

强效温室气体甲烷（CH₄）的全球大气浓度正快速攀升，农业贡献了每年30%~50%的甲烷排放量。为遏制其全球变暖效应，亟需实施强有力且持续的减排措施。此前已有实验室研究证实，堆肥等有机改良剂（organic amendments）类的可持续农业管理策略，可有效实现甲烷减排。本研究通过大规模野外实地研究，探究了有机改良剂对农田土壤甲烷减排潜力以及甲烷循环微生物群落的影响。结果显示，施加有机改良剂的土壤具有更高的甲烷潜在吸收速率，且将甲烷氧化至亚大气浓度的能力得到提升。此外，本研究首次明确证实，施加有机改良剂后，农田土壤的甲烷氧化菌（methanotrophic）与产甲烷菌（methanogenic）群落不仅丰度提升，多样性反而降低，群落结构发生显著改变。堆肥改良的土壤……
# 堆肥施加提升农田土壤甲烷减排潜力并调控甲烷循环微生物群落
数据集DOI：[10.5061/dryad.dz08kps8h](10.5061/dryad.dz08kps8h)

## 数据与文件结构说明
为评估有机施肥对农田土壤甲烷吸收潜力及甲烷循环微生物群落的影响，本研究开展了大规模野外实地筛查。
为此，我们在荷兰、比利时与瑞士的6个采样点采集了0~15cm深度的土壤样品。测定的理化性质包括：含水率、有机质含量、pH值、硝态氮+亚硝态氮（NO₃+NO₂-N）、铵态氮（NH₄-N），以及铝（Al）、钴（Co）、铜（Cu）、铁（Fe）、钾（K）、镁（Mg）、锰（Mn）、钼（Mo）、钠（Na）、镍（Ni）、磷（P）、硫（S）与锌（Zn）含量。此外，我们还在顶空甲烷浓度为10或10000 ppmv的培养体系中，测定了土壤的潜在甲烷吸收速率与滞后期，以及将甲烷氧化至亚大气浓度的能力。进一步检测了细菌16S rRNA、*pmo…*的基因拷贝数……