Data and code for: Somatostatin peptide signaling dampens cortical circuits and promotes exploratory behavior

Our dataset reflects the data and code used in Brockway et al., Cell Reports. We sought to characterize the unique role of somatostatin (SST) in the prelimbic (PL) cortex. We perform slice electrophysiology in pyramidal and GABAergic neurons to characterize the pharmacological mechanism of SST signaling, and fiber photometry of GCaMP6f fluorescent calcium signals from SST neurons to characterize the activity profile of SST neurons during exploration of an elevated plus maze (EPM) and open field test (OFT). We used local delivery of a broad SST receptor (SSTR) agonist and antagonist to test causal effects of SST signaling. SSTR activation hyperpolarizes layer 2/3 pyramidal neurons, an effect that is recapitulated with optogenetic stimulation of SST neurons. SST neurons in PL are activated during EPM and OFT exploration, and SSTR agonist administration directly into the PL enhances open-arm exploration in the EPM. This work describes a broad ability for SST peptide signaling to modulate m..., ,

本数据集对应Brockway等人发表于《Cell Reports》的研究中所使用的实验数据与代码。本研究旨在明确生长抑素（somatostatin, SST）在前边缘皮层（prelimbic cortex, PL）中的独特功能。我们通过对锥体神经元与γ-氨基丁酸能（GABAergic）神经元开展脑片电生理实验，以解析SST信号通路的药理学机制；同时通过光纤光度法记录SST神经元的GCaMP6f荧光钙信号，以刻画SST神经元在高架十字迷宫（elevated plus maze, EPM）与旷场实验（open field test, OFT）探索过程中的活动特征。我们通过局部递送广谱生长抑素受体（somatostatin receptor, SSTR）激动剂与拮抗剂，验证SST信号通路的因果调控效应。激活SSTR可使2/3层锥体神经元发生超极化，该效应可通过光遗传刺激SST神经元得以重现。前边缘皮层内的SST神经元会在高架十字迷宫与旷场探索过程中被激活，且直接向前边缘皮层局部递送SSTR激动剂，可增强小鼠在高架十字迷宫中的开放臂探索行为。本研究揭示了SST肽类信号通路具备广泛调控……的能力。