Sexually dimorphic ozone-induced changes in fetal rat hypothalamus

IUGR is associated with an increased risk of obesity in offspring, which is likely driven in part by reprogramming of the hypothalamus due to suboptimal nutrition and hypoxia during development. As such, we performed mRNA-seq on whole hypothalamic tissue to identify altered expression of genes in our model of IUGR and to identify major pathways that could be associated with fetal undernutrition near term. Overall design: Kidneys from 3 naïve adult male Sprague-Dawley rats, 6 months old, were collected and laser capture dissection was used to sample the proximal tubules, glomeruli, the thick ascending limb (TAL) of the loop of Henle, and collecting duct (CD). Samples were measured for microRNAs using small RNA sequencing on an Illumina HiSeq 2000.