Full data set of publication "Enhancing the performance of Magnets photosensors"

The data set characterizes variants of the nMag/pMag photodimerization system, which were generated through random mutagenesis coupled to high-throughput screening to adjust light sensitivity and activation. Variants were characterized in vivo in bacteria by flow cytometry and during the entire growth curve by spectrofluorometry. Mutations were identified that either increase or decrease the light sensitivity at sub-saturating light intensities, while also improving the light activation and dark-to-light fold change.该数据集表征了nMag/pMag光二聚化系统（nMag/pMag photodimerization system）的各类变体。上述变体通过随机诱变结合高通量筛选技术构建，用于调控其光敏感性与光激活特性。研究人员借助流式细胞术在细菌体内完成变体表征，并通过荧光分光光度法在完整生长曲线周期内开展检测。研究人员已鉴定出可在亚饱和光强下提升或降低光敏感性的突变位点，同时此类突变还可优化光激活性能以及暗态至光态的信号倍数变化。