RNA-Seq on Primary human hepatocytes Control
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After extractions, cells were cultured in 5% CO2 at 37°C using Williams E media supplemented with 2.24 g/l NaHCO3, 100 U/100 µM penicillin/streptomycin, 100 µM dexamethasone and 2 mM L-glutamine. RNA was isolated using the RNeasy® Mini Kit (Qiagen, 74104) on-column DNA digestion was performed. Libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit (New England Biolabs GmbH, Germany) Genome assembly: hg38
细胞提取完成后，于37℃、5%二氧化碳（CO₂）培养条件下，使用添加了2.24 g/L 碳酸氢钠（NaHCO₃）、100 U/100 μM 青霉素-链霉素、100 μM 地塞米松以及2 mM L-谷氨酰胺的Williams E培养基进行细胞培养。 采用RNeasy® Mini试剂盒（Qiagen，货号74104）提取总RNA，并同步完成柱上DNA消化处理。 使用NEBNext Ultra II RNA文库制备试剂盒（New England Biolabs GmbH，德国）构建测序文库。 基因组组装版本：hg38
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Zenodo创建时间：
2024-01-19
