Description of all single-base mutations observed for each of the 50 lines of the 4 strains in the 200-day experiment.

The table describes the position of the mutations in the (single contig) assembly of the ancestral strain, the inferred single base change, the category of site, whether or not the site is shared across strains (‘Shared region’), whether the site is on the leading or lagging strand, the two flanking bases, and whether or not the mutation is clustered (within 30 bp of another mutation in that line). The site categories are 1st/2nd codon positions (1), 3rd codon positions (3), 4-fold degenerate sites (4) and intergenic (0). Shared sites (1) are defined by whether or not the gene is present in all strains for sites within genes, and whether or not the two flanking genes are present in all strains for intergenic regions. (XLSX)