Description of all single-base mutations observed for each of the 50 lines of the 4 strains in the 200-day experiment.

The table describes the position of the mutations in the (single contig) assembly of the ancestral strain, the inferred single base change, the category of site, whether or not the site is shared across strains (‘Shared region’), whether the site is on the leading or lagging strand, the two flanking bases, and whether or not the mutation is clustered (within 30 bp of another mutation in that line). The site categories are 1st/2nd codon positions (1), 3rd codon positions (3), 4-fold degenerate sites (4) and intergenic (0). Shared sites (1) are defined by whether or not the gene is present in all strains for sites within genes, and whether or not the two flanking genes are present in all strains for intergenic regions. (XLSX)本表格详述了祖先毒株的单连续基因组片段（contig）组装序列中突变的位置、推断的单碱基替换类型、位点类别、该位点是否跨毒株共享（即"共享区域"）、该位点处于前导链还是滞后链、两侧侧翼碱基，以及该突变是否成簇（即该品系内存在其他突变且二者间距不超过30 bp）。位点类别分为密码子第1/2位（标记为1）、密码子第3位（标记为3）、4倍简并位点（标记为4）以及基因间区（标记为0）。共享位点（标记为1）的判定标准为：若位点位于基因内部，则需该基因在所有毒株中均存在；若位点位于基因间区，则需其两侧的两个基因在所有毒株中均存在。（XLSX）