Effects of Calcium Hypochlorite and Octenidine Hydrochloride on L929 And Human Periodontal Ligament Cells

Abstract The aim of this study was to assess cytotoxicity and cell migration of calcium hypochlorite [Ca(OCl)2] and octenidine hydrochloride - OCT (Octenisept®, Schülke & Mayr, Norderstedt, Germany) in L929 and human periodontal ligament (hPDL) cells. The cells were exposed to different doses of different solutions: 2.5% and 5% Ca(OCl)2, 0.1% OCT, 2.5% NaOCl and 2% CHX for 10 min. Cell viability was assessed by methyl-thiazol-tetrazolium (MTT) and neutral red (NR) assays, and cell migration was determined by wound-healing assay. Statistical analysis was performed by two-way ANOVA and Bonferroni tests (α=0.05). The MTT and NR assays revealed that 0.1% OCT was less cytotoxic in hPDL cells (p0.05), but these solutions showed greater cytotoxicity than the others. The result was the same for L929 cells, except that there was no significant difference between 2% CHX and 2.5% Ca(OCl)2 (p>0.05). Wound-healing assay in L929 and hPDL cells showed that cell migration of 0.1% OCT, 2% CHX and 2.5% Ca(OCl)2 groups was higher than 5% Ca(OCl)2 and 2.5% NaOCl groups at 24 h (p摘要 本研究旨在评估次氯酸钙[Ca(OCl)₂]与盐酸奥替尼啶（OCT，Octenisept®，Schülke & Mayr，德国北奈施泰特）对L929细胞及人类牙周膜（hPDL）细胞的细胞毒性与细胞迁移能力。将细胞暴露于不同浓度的多种溶液中10分钟，包括2.5%与5%次氯酸钙、0.1% OCT、2.5%次氯酸钠（NaOCl）以及2%氯己定（CHX）。采用噻唑蓝（MTT）法与中性红（NR）法检测细胞活力，通过划痕愈合实验测定细胞迁移能力。统计学分析采用双因素方差分析与邦费罗尼检验（α=0.05）。MTT与NR检测结果显示，0.1% OCT在hPDL细胞中细胞毒性更低（p0.05），但上述受试溶液的细胞毒性均高于其余组别。L929细胞的实验结果与此一致，仅2% CHX与2.5% Ca(OCl)₂组间无显著性差异（p>0.05）。对L929与hPDL细胞的划痕愈合实验结果显示，培养24小时时，0.1% OCT、2% CHX及2.5% Ca(OCl)₂组的细胞迁移能力高于5% Ca(OCl)₂与2.5% NaOCl组（p