Jennifer Bestman, Holly Cline (2011) CIL:37016, Xenopus laevis, CNS neuron (sensu Vertebrata). CIL. Dataset

This is IMAGE #3 (day 3) of a time lapse series contained with in this image group. It is one of two images of a pair of neuron from the optic tectum of an albino Xenopus laevis tadpole CNS, stage 47 at this timepoint.

The images were acquired daily over 3 days and show dendritic arbor development and morphological plasticity of the dendritic arbor with branch additions and retractions.

The cells were transfected with in vivo electroporation of plasmid DNA encoding eYFP ~24 hours before the first image was acquired. The intact, living tadpole was anesthetized, positioned under a glass coverslip and this image was taken directly through its transparent skin using a custom built two-photon microscope.

本图组中包含一组时间序列照片的第3张图像（第3天）。该图像为一对来自白化非洲爪蟾（Xenopus laevis）中枢神经系统视顶盖神经元的一对图像之一，拍摄于47期这一时间点。该图像系列每日采集，持续3天，展示了树突枝芽的生长发育以及树突枝芽的形态可塑性，包括分支的增加与退缩。在采集第一张图像前约24小时，通过原位电穿孔法将编码eYFP的质粒DNA转染至细胞中。在采集图像前，活体蝌蚪经过麻醉处理，置于载玻片下，并通过定制建造的双光子显微镜直接穿过其透明的皮肤进行拍摄。